PROCESS FOR PRODUCING HUMAN LEUCOSITE AND HUMAN GAMMA INTERFERONS IN THE SAME CULTURE IN CONSECUTIVE STEPS

摘要

The invention relates to a process for the preparation of alpha - and gamma -interferon by isolating the buffy coat fraction of blood, removing the erythrocytes, suspending the leukocytes in a suitable nutrient medium and treating with an alpha -interferon inducer and with a gamma -interferon inducer, which comprises producing alpha -interferon and gamma -interferon one after the other in the same leukocyte culture by pre-treating the suspension obtained after the separation of the leukocyte/buffy coat/fraction of blood, removal of the erythrocytes and suspending the leukocytes in a suitable nutrient medium with - or beta -interferon, contacting with an alpha -interferon inducer, separating the liquid containing the alpha -interferon from the cells, if desired recovering the -interferon, washing and suspending the cells in a suitable nutrient medium, treating with a mitogenic agent, separating the liquid comprising alpha -interferon from the cells and if desired recovering alpha -interferon and if desired making alpha -interferon free of alpha -interferon. The advantage of the process is that alpha - and gamma -interferon are produced from the same leukocyte culture in two steps. This makes the process more economical and enables an effective utilization of the limitedly available leukocytes.