GENETICALLY MODIFIED PLANT CELLS USING T-DNA FROM AGROBACTERIUM

摘要

Disarming mutations of tmr and tms T-DNA genes are disclosed which are useful in an Agrobacterium tumefaciens Ti plasmid-based plant transformation vector. As disarmed T-DNA is compatible with standard plant regeneration protocols, such a vector may be used to introduce a plant-expressible foreign gene into a dicot plant. Methods for use of disarmed T-DNA are provided as are morphologically normal transformed plants. The invention is exemplified by introduction of a Phaseolus vulgaris lectin gene into tobacco.