PRODUCTION OF SAPONIN CONTAINING NO ISOFLAVONE FROM SOYBEAN EMBRO BUD

摘要

PURPOSE:To obtain saponin of high purity without containing isoflavones on an industrial scale, by adding water to an extract concentrate of soybean embryo buds with a lower alcohol, adsorbing the extract on an adsorbent, eluting the adsorbed extract stepwise with a hydrophilic organic solvent and purifying the eluate by gel filtration, etc. CONSTITUTION:Soybean embryo buds are extracted with a lower alcohol and concentrated, and water is then added thereto. The resultant extract is then adsorbed on an adsorbent, e.g. HP-20, HP-40, etc., and isoflavones are mostly washed and removed with 10-40% methanol, ethanol, acetone, etc. The adsorbed extract is subsequently eluted with 70-80% methanol, ethanol, etc., to give an eluate, which is then purified by gel filtration and partition chromatography to afford the aimed saponin containing no isoflavones. Thereby, the isoflavones giving side effect to the human body are removed from the soybean embryo buds with a higher saponin content than soybean flour and the aimed saponin of high purity can be obtained in large amounts industrially.