Method for preparing sluggish vaccine against ' AUJEZSKY ' disease

摘要

A vaccine against Aujeszky disease is produced by inoculating a cell culture, preferably of primary chicken embryo cells or primary calf testicle monolayer cells, with a virulent strain of the virus deposited in the Hungarian Institute of Public Health under the number 00236/82. The virus used to inoculate the cell culture is preferably obtained from a suspension of the brain of a rabbit that contracted the said strain of Aujeszky disease. The virus propagated in the cell culture is then inactivated, preferably with ethylene imine, optionally obtained by cyclising bromoethyl amine immediately prior to use, and then mixed with an adjuvant, which is advantageously an oily adjuvant e.g. Phylaxia incompletely oily immunoadjuvant. The vaccine is effective to protect livestock and domestic animals from Aujeszky disease.