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Method for the production of heterologous proteins by Yarrowia lipolytica transformants, method for the production and detection of Yarrowia lipolytica transformants and method for the manufacture of transformants for usable plasmids
Method for the production of heterologous proteins by Yarrowia lipolytica transformants, method for the production and detection of Yarrowia lipolytica transformants and method for the manufacture of transformants for usable plasmids
Sequencing of the XPR2 and LEU2 genes of Yarrowia lipolytica, recombinant Yarrowia lipolytica cloning vehicles comprising heterologous DNA coding for the expression of mammalian protein and other polypeptides, including plasmids suited for transformation of Y. lipolytica hosts and incorporating a regulon homologous to the host in its untransformed state, and secretion signals for the heterologous gene; integrative expression vectors using the XPR 2 gene promoter, alkaline protease pre-proregion and XPR2 terminator region and those having the LEU2 promoter and alkaline protease secretory signal sequences capable, in a transformed Y. lipolytica cell culture, of expressing and secreting a heterologous protein outside the cell; Y. lipolytica transformants comprising said vectors and plasmids; methods for preparing vectors to direct secretion of specified heterologous proteins coded for by genes, cDNA or synthetic DNA in Y. lipolytica in their mature, functional state.
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