A PROCESS FOR THE PREPARATION OF ESTRIOL 3-0-CARBOXYMETHYL ENTHERGLUCOSE 6-PHOSPHATE DEHYDROGENASE ENZYME CONJUGATES

摘要

A process for the preparation of estriol glucose-6-phosphate dehydrogenase conjugate comprising reacting N-hydroxysuccinimide and dicyclohexyl carbodimide with estriol-3-carboxymethyl ether in the presence of anhydrous organic solvent and further reacting the reaction mixture with glucose-6-phosphate dehydrogenase and the enzyme conjugate formed is separated from the reaction mixture by chromatography and lyophillized.