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Selecting genetically transformed plant cells - using marker including nucleotide sequence conferring resistance to bleomycin or related cpds.
Selecting genetically transformed plant cells - using marker including nucleotide sequence conferring resistance to bleomycin or related cpds.
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机译:选择遗传转化的植物细胞-使用包括赋予博来霉素或相关cpds抗性的核苷酸序列的标记。
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摘要
Selection of genetically transformed plant cells from mixts. with non-transformed plant cells is effected by contacting the mixt. with a substance (I) which selectively attacks the non-transformed cells. (I) is fleomycin, bleomycin, tallysomycin and/or a substance with a similar structure, and the DNA introduced into the transformed cells contains a marker including a nucleotide sequence conferring resistance to (I). The marker is specifically one known to impart resistance to (I) in bacterial cells, e.g. the bleomycin resistance marker in the Tn5 transposon of E.coli.
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