Free-flow electrophoretic separation method and apparatus therefor

摘要

The invention discloses a free-flow electrophoretic separation method and apparatus for separating charged substances such as proteins, cells, and the like, by electrophoresis, which comprises supplying a mixed solution containing the charged substances to be separated, which are dissolved in a separation buffer, into a separation chamber (1), circulating the mixed solution inside the separation chamber (1), applying a d.c. voltage to the mixed solution to cause the electrophoresis of the charged substances and to separate them, and withdrawing the charged substances thus separated from outlets (10, 11) disposed at one and the other end of the separation chamber (1).