Method of extraction of a protein leaf from a material vegetale and more particularly of a plant type nicotiana

摘要

Method of extraction of a protein of the leaves of plants, more particularly of the genus nicotiana in which: / p & & p & a. it is reduced, the sheets in the pulp, for example, in the presence of 0 to 2,5fois the weight of starting green of water and of 0,5 has 2parties of an anti - oxidizing agent for 100parties vegetale of material; / p & & p & b. the mixture is extracted immediately of the mixture obtained in the liquid, or juice, green, containing the major part of a protein which are insoluble in suspension and of a protein soluble and / p & & p & is done precipitate the insoluble proteins; / p & & p & d.on separate the precipitate of the liquid in order to clarify this liquid fraction containing a i and ii the proteinic fraction / p & & p & e. it is precipitate the protein fraction i of the liquid clarified resultant of the step previous, and / p & & p & f.on extract the protein fraction i thus obtained, characterizes by the fact that, in order to make precipitate the protein fraction i, the partitions of the carbon dioxide co in the gaseous state in the clarified liquid, at least up to saturation of the liquid co.