Method of and apparatus for carrying out separation analysis investigations in a planar system

摘要

The method includes carrying out simultaneously or separately on-line chromatographic and/or electrophoretic measurements, of constituents of a complex mixture fed to a planar plate covered by a sorbent layer being the place of separation, continuing the on-line measurement up to reaching a given value limit following from the applied retention mechanism and then carrying out off-line measurements of constituents remaining on the separation plate. The apparatus includes at least one separation plate (18) covered by a sorbent layer (19), means for forcing flow of a carrier medium on the separation plate (18), means for feeding the carrier medium into the sorbent layer (19), means (22) for introducing a sample of a complex mixture into the sorbent layer (19), means (23) for conducting fluid medium from at least one edge of the separation plate (18), a detector unit (E) (suitably a flow chamber detector) including at least one element for real-time measurements of the fluid medium, and a sensing unit (F) (suitably a densitometer) arranged oppositely to the surface of the separation plate (18) covered by the sorbent layer (19). IMAGE