IMMUNOLOGICAL DETECTION METHOD OF MYCOBACTERIUM IN PHLEGM, MONOCHRONAL ANTIBODY USED FOR SAID METHOD AND HYBRIDOMA CELL LINE PRODUCING SAID MONOCHRONAL ANTIBODY

摘要

Mycobacteria can be directly detected in sputum when a liquefied sputum sample is subjected to treatment with heat and surfactant for 2 - 4 minutes, the sputum sample treated in this way is smeared, the smear is incubated with a labelled polyclonal or monoclonal antibody against mycobacteria in the presence of moisture, and the sample is subjected to an immunological detection method suitable for the label. Particularly suitable monoclonal antibodies are derived from hybridoma cell lines BS 100, 102, 104 and 107.