首页> 外国专利> METHOD AND APPARATUS FOR DETECTING DEOXYRIBONUCLEIC ACID AND RIBONUCLEIC ACID TYPE VIRAL GENOM IN BIO-CULTURE MEDIUM, ESPECIALLY, IN SERUM

METHOD AND APPARATUS FOR DETECTING DEOXYRIBONUCLEIC ACID AND RIBONUCLEIC ACID TYPE VIRAL GENOM IN BIO-CULTURE MEDIUM, ESPECIALLY, IN SERUM

机译:检测血清,生物培养基中脱氧核糖核酸和核糖核酸型病毒基因组的方法和装置

摘要

A sample of biological medium liable to be contaminated by viral particles is first of all treated to digest the nonviral DNA and RNA nucleic acids and to preserve the viral nucleic acids. The sample is then purified, if appropriate, for example by being passed through a cartridge (32) containing a gel for retaining the proteins present in the sample. The viral nucleic acids are then extracted and denatured in the presence of an extraction medium in a storage vessel (62). They are then collected and then detected by an appropriate process, for example by a colorimetric process. The invention makes it possible to carry out in a particularly simple manner the polyspecific detection of the viral genomes containing DNA and RNA, especially in biological media such as serum. IMAGE
机译:首先处理易于被病毒颗粒污染的生物培养基样品,以消化非病毒DNA和RNA核酸并保存病毒核酸。然后将样品纯化,如果合适的话,例如通过使其通过含有用于保留样品中存在的蛋白质的凝胶的药筒(32)。然后在储存容器中在提取培养基的存在下提取病毒核酸并使其变性(62)。然后收集它们,然后通过适当的过程(例如,比色过程)进行检测。本发明使得可以以特别简单的方式对包含DNA和RNA的病毒基因组进行多特异性检测,尤其是在诸如血清的生物培养基中。 <图像>

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