A method for measuring the level of antigens or antibodies in the circuit, and method for measuring the level of haptens

摘要

A method for measuring the level of circulating antigen (Ag1), antibody (Ab1) or hapten (H) in a liquid sample, which comprises forming in a liquid phase reaction a soluble complex wherein said antigen (Ag1) antibody (Ab1) or hapten (H) is linked through, respectively, a specific antibody (Ab), antigen (Ag) or antihapten (Anti-H), to a matrix which is soluble in the liquid phase and carries a ligand (X), subsequently forming an insolubilised complex comprising a solid support linked to the ligand (X) of said soluble complex through an anti-ligand (Y), the insolubilised complex carrying a label (Z) linked to the antigen (Ag1) through an anti-antigen (Anti-Ag1), to the antibody (Ab1) through an anti-antibody (Anti-Ab1) or to the hapten (H), washing the insolubilised complex, and checking the washed insolubilised complex for the presence of the label (Z) to provide a measure of the level of said antigen (Ag1), antibody (Ab1) or hapten (H) in said sample.