Growth factor, nucleotide sequence encoding the growth factor expression plasmid comprising the nucleotide sequence, the plasmid containing host protein produced by the host, method of preparation of the growth factor and the pharmaceutical preparation containing the growth factor

摘要

Unique genes coding for the amino acid sequence of bovine acidic fibroblast growth factor (aFGF) and human aFGF are constructed. The bovine gene is derived from reverse translation of the aFGF amino acid sequence while the human gene is derived by specific point mutations of the bovine gene. Each gene construct is inserted into an expression vector which is used to transform an appropriate host. The transformed host cells produce recombinant aFGF (r-aFGF), human or bovine, which is purified and has an activity equivalent to the native protein.