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UTTRYCKANDE AV PLASMIDER INNEHAOLLANDE DEO-PROMOTORN OCH BAKTERIEMODERCELLER INNEHAOLLANDE DESSA PLASMIDER.
UTTRYCKANDE AV PLASMIDER INNEHAOLLANDE DEO-PROMOTORN OCH BAKTERIEMODERCELLER INNEHAOLLANDE DESSA PLASMIDER.
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机译:包含DEO启动子的质粒和包含这些质粒的细菌母细胞的表达。
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摘要
Plasmids are provided which upon introduction into a suitable Escherichia coli host render the host capable of effecting expression of DNA encoding a desired naturally-occurring polypeptide or polypeptide analog thereof under the control of the deo P1-P2 promoter. Further plasmids are also provided which thermoinducibly direct expression of eucaryotic genes under the control of a lambda PL promoter and a thermolabile repressor protein present on the same plasmid under the control of a deo P1 promoter. These plasmids have been inserted into various hosts, some of which contain DNA encoding the repressor protein. Such plasmids may be used to produce polypeptides such as human copper/zinc superoxide dismutase, hMnSOD-hGPX fusion polypeptides, human manganese superoxide dismutase, human growth hormone, bovine growth, porcine growth, human growth hormone-apolipoprotein E fused polypeptides, apolipoprotein E and various fibronectin domains.
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