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A recombinant DNA expression vector encoding deacetoxysepheporosporin C synthase and deacetyl cephalosporin C synthase and a DNA compound
A recombinant DNA expression vector encoding deacetoxysepheporosporin C synthase and deacetyl cephalosporin C synthase and a DNA compound
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机译:编码脱乙酰氧基丝孢菌素C合酶和脱乙酰头孢菌素C合酶的重组DNA表达载体和一种DNA化合物
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摘要
The present invention relates to DNA compounds and expression vectors that encode deacetoxycephalosporin C synthetase (DAOCS) and deacetylcephalosporin C synthetase (DACS) activities. The compounds can be used to construct recombinant DNA expression vectors for a wide variety of host cells, including E. coli, Penicillium, and Cephalosporium. The invention also relates to the regulatory elements of the Cephalosporium acremonium DACS/DAOCS gene. A method for selecting Penicillium transformants, including those able to synthesize cephalosporin antibiotic due to the presence of the DACS/DAOCS-encoding DNA, is also provided. This transformation system utilizes an acetamidase gene for purposes of selecting transformants.
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