METHOD OF DETECTING BACTERIA-CARRIAGE OF TYPHOID FEVER INFECTION

摘要

The invention relates to medicine, not specifically to the diagnosis of infectious diseases, intended for the detection of latent bacterial carriage. The purpose of the invention is to improve the accuracy of detecting hidden forms of abdominal typhoid carriage during mass examinations of the population in an atraumatic manner. To do this, treated with 0.1% p-rum of bovine serum albumin, dried and methanol-fixed coverslips (10 pcs.) Applied urine to be tested. The drops are dried in a stream of warm air and fixed for 10 minutes with methanol. Rabbit serum, specific to the L-forms of typhoid bacteria (at a dilution of 1: 1280), is applied to smears washed from salts. The glasses are placed in a humid chamber, incubated for 30 minutes at 37 ° C, washed and dried. Then anti-rabbit antibodies with a radioactive isotope introduced into their composition (diluted 1: 100) are applied to smears. Calculate the radioactivity of each sample. In a v-spectrometer. Similarly and simultaneously, the analysis of control urine samples of obviously healthy people is performed. Results are statistically processed. e (L with with NU i with