Method of preparation of a strain, in particular of yeast, transformed by an expression vector, which may be cultivated on a complete medium without pressure of the selection and strain thus obtained

摘要

Process for the preparation of a strain transformed by a cloning or expression vector of a gene coding for a predetermined protein which may be cultivated on a complete medium without selection pressure, characterized in that: a) a metabolite of which the absence is lethal for the strain and which is synthetized by at least two metabolic pathways is identified, b) a first pathway of said metabolic pathways is blocked by alteration of at least one of the genes implied in said pathway, c) the strain is transformed by the vector which additionally comprises the functional allele of the altered gene, and d) the other metabolic pathways are blocked.