PURPOSE:To enable promotion of growth rate of seedlings and remarkable shortening of culture period, by hermetically sealing a culture atmosphere regulator consisting of a gaseous carbon dioxide substitution type disoxidizer coexisting together with a culture vessel containing plant tissues bedded therein. CONSTITUTION:A gaseous carbon dioxide substitution type disoxidizer, containing an iron based oxygen absorber, such as reduced iron powder or ferrous sulfate, hygroscopic or deliquescent alkaline earth metal salt, such as calcium chloride, a carbonate and/or hydrogencarbonate, such as sodium carbonate, and a solid substance, such as benzoic acid, reactive with water to assume acidity as essential components and capable of absorbing oxygen and generating gaseous carbon dioxide is prepared to provide a culture atmosphere regulator. The resultant regulator, together with a growth point cut at the top of a stem and a culture vessel used for germ-free culture in a culture medium, etc., are then hermetically sealed to exist together in a light-transmitting and air- impermeable vessel, such as polyvinylidene chloride-coated film to culture the plant tissues. Thereby, growth of the culture seedlings is promoted to remarkably shorten the culture period. Equipment cost is also reduced.
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