A process for the preparation of an enzymatic protein hydro lysate, rich in di - and tripeptides, be used in particular in the case of the art nutrition and in the dietetics.

摘要

Process for the enzymatic hydrolysis of proteins, employing a combination of the following three enzymes added simultaneously or successively: a) a bacterial protease which is usable in a medium in the vicinity of neutrality (pH 5 to 8), extracted from strains of Bacillus subtilis and chosen from the group comprising Neutrase TM , Protéase B500 TM and H.T. Protéolytique TM , b) a bacterial protease which is usable in an alkaline medium (pH 7 to 11), extracted from strains of Bacillus licheniformis or Bacillus subtilis and chosen from the group comprising Alcalase TM , Protéase A2 TM and Optimase TM , and c) a pancreatic enzyme of animal origin, including trypsin or a trypsin concentrate or the product marketed under the name PEM TM , under the following conditions of hydrolysis: - a neutral to alkaline pH of 7 to 10, - a temperature of 20 to 70 DEG C, and preferably 30 to 50 DEG C, - a hydrolysis period preferably not exceeding 300 minutes. Such a process enables hydrolysates rich in di- and tripeptides and having a low content of free amino acids to be obtained, for use, in particular, in artificial nutrition and in dietetics.