EXPRESSION OF RECOMBINANT GENE, EXPRESSIVE VECTOR AND EXPRESSIVE ACCESSORY VECTOR

摘要

To express a recombinant gene which contains AGA and/or AGG codons for arginine in E. coli after transformation with an expression vector which contains the recombinant gene, the amount of t-RNA which incorporates arginine and recognises the codons AGG and AGA and which is present in the E. coli cells is increased to not less than five times the amount normally occurring in these cells, or a strain which contains an amount of t-RNA which recognises the codons AGG and AGA and incorporates arginine which is not less than five times the amount normally occurring in E. coli is selected, and this strain is used as host cells. Increase in the vitality of rec-t-PA-producing E. coli by the expression assisting plasmid pUBS 500 IMAGE t (h after induction).