首页>
外国专利>
HOMOGENEOUS competitive binding immunoassay FOR DETERMINATION OF A iodothyronine IN A TEST OF unextracted SERUM OR PLASMA WITH TBP-BLOCKING AGENT AND reagent FOR USE IN THE METHOD
HOMOGENEOUS competitive binding immunoassay FOR DETERMINATION OF A iodothyronine IN A TEST OF unextracted SERUM OR PLASMA WITH TBP-BLOCKING AGENT AND reagent FOR USE IN THE METHOD
展开▼
机译:用TBP阻滞剂和试剂进行均相竞争结合免疫测定法测定未提取血清或血浆中的碘甲状腺素
展开▼
页面导航
摘要
著录项
相似文献
摘要
An improved immunoassay method, reagent means, and test kit for determining an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or plasma, wherein fenclofenac and related phenylacetic acids, or salts thereof, are employed as novel blocking agents for the binding of iodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays wherein a spectrophotometric response is generated in the assay reaction mixture at a wavelength greater than about 300 nm, the blocking agents of the present invention having been found to have no substantial absorbance at wavelengths above 300 nm. Such homogeneous immunoassays include those which employ labels such as fluorescers, enzyme substrates, enzyme prosthetic groups, enzymes, and enzyme inhibitors.
展开▼