首页> 外国专利> SPECIES-SPECIFIC DNA PROBE FOR THE DETECTION OF $i(BRANHAMELLA CATARRHALIS)

SPECIES-SPECIFIC DNA PROBE FOR THE DETECTION OF $i(BRANHAMELLA CATARRHALIS)

机译:用于检测$ i(布拉汉姆氏酵母)的特定物种DNA探针

摘要

There is provided by the present invention, a method for detecting the presence of Branhamella catarrhalis, said method comprising, depositing and fixing on an inert support a sample containing DNA fragments in substantially single stranded form, contacting said fixed single stranded genetic material with a labelled probe consisting of a fragment of B. catarrhalis chromosomal DNA, identified as ATCC 53879, being capable of hybridizing with B. catarrhalis DNA in the sample, said contacting being under hybridizing conditions at a pre-determined stringency; and detectiing duplex formation on said support by means of said label.
机译:本发明提供了一种用于检测卡他氏杆菌的存在的方法,所述方法包括将包含基本上单链形式的DNA片段的样品沉积并固定在惰性载体上,使所述固定的单链遗传物质与标记的DNA接触。探针由卡他芽孢杆菌染色体DNA的片段组成,鉴定为ATCC 53879,能够与样品中的卡他芽孢杆菌DNA杂交,所述接触在杂交条件下以预定的严格性进行;通过所述标记检测在所述载体上的双链体形成。

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