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PREPARATION OF ANTIHEMOPHILIC FACTOR, ANTIHEMOPHILIC FACTOR PREPARED THEREWITH, AND MEDICINE COMPOSITION CONTAINING SAID ANTIHEMOPHILIC FACTOR
PREPARATION OF ANTIHEMOPHILIC FACTOR, ANTIHEMOPHILIC FACTOR PREPARED THEREWITH, AND MEDICINE COMPOSITION CONTAINING SAID ANTIHEMOPHILIC FACTOR
The invention relates to a process for preparing very pure antihaemophilic factor (FVIIIc). This process for preparing very pure antihaemophilic factor (FVIIIc) predominantly devoid of von Willebrand factor, comprising a step of purification by ion exchange chromatography using a chromatography column comprising a gel, comprising a step of adsorption of the antihaemophilic factor on the gel of the aforementioned column and a step of desorption of the purified antihaemophilic factor which is collected, is characterised. An antihaemophilic factor devoid of the majority of the von Willebrand factor and having an activity as high as 250 IU/mg of proteins is thereby obtained.
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机译:本发明涉及制备非常纯的抗血友病因子(FVIIIc)的方法。该制备主要不含von Willebrand因子的非常纯的抗血友病因子(FVIIIc)的方法,包括使用含有凝胶的色谱柱通过离子交换色谱法纯化的步骤,该步骤包括将抗血友病因子吸附在上述凝胶上的步骤表征柱子和收集的纯化抗血友病因子的解吸步骤。由此获得了缺乏大多数von Willebrand因子并且具有高达250 IU / mg蛋白质活性的抗血友病因子。
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