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DETERMINATION OF DEACETYLATION DEGREE OF CHITIN AND CHITOSAN
DETERMINATION OF DEACETYLATION DEGREE OF CHITIN AND CHITOSAN
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机译:甲壳素和壳聚糖脱乙酰度的测定
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摘要
PURPOSE:To easily and accurately determine the deacetylation degree of chitin and chitosan by completely decomposing chitin or chitosan with an enzyme to the constituent monosaccharides and quantitatively determining the monosaccharides. CONSTITUTION:Chitin or chitosan is decomposed into monosaccharides (Nacetyl glucosamine and glucosamine) by adding exo-type beta-D-glucosaminidase in combination with beta-N-acetylhexosaminidase (or beta-N-acetylglucosaminidase) and, as necessary, chitosanase, chitinase, etc., to a solution of chitin or chitosan. The formed monosaccharides are quantitatively determined by high performance liquid chromatography, etc., to determine the objective deacetylation degree.
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