PURPOSE:To massively produce an Acanthopanax senticosus tissue cultured product containing physiologically active substances in the same amounts as the natural Acanthopanax senticosus by culturing a callus derived from the plant tissue of the Acanthopanax senticosus in a medium containing ammonium sulfate. CONSTITUTION:The plant tissue of Acanthopanax senticosus is sterilized with an alcohol, antiformin, etc., embedded aseptically in a nutritive agar medium containing auxin and cytokinin and subsequently cultured at 23-27 deg.C in a dark state. A callus is generated from the small piece of the tissue and subcultured in the same medium until the growth of a size larger 3-4 times than the original size for 4 weeks. The calluses are subsequently cultured in a medium containing ammonium sulfate preferably in an amount of 100-200mg/l, such as Gamborg B-5 medium at 23-27 deg.C to produce the Acanthopanax senticosus tissue cultured product containing eleutherosides and physiologically active substances such as isofraxidin and chlorogenic acid in the same or more amounts as the natural Acanthopanax senticosus.
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