ENZYME ACTIVITY ASSAY EMPLOYING A RECEPTOR SPECIFIC FOR THE PRODUCT OR SUBSTRATE TO FORM DISTINGUISHABLE COMPLEXES DETECTABLE BY A BIOLUMINESCENT PROTEIN LABEL

摘要

A method for determining the level of activity of an enzyme in a sample, comprising combining in a reaction medium a sample suspected of containing the enzyme and a labeled substrate for the enzyme capable of reacting with the enzyme to provide a product; allowing a reaction to take place between the enzyme and the substrate, whereby the product is formed in the presence of the enzyme; combining with the reaction medium, either before or after the reaction is allowed to take place, a receptor capable of binding specifically with either the labeled product or the labeled substrate, but not both, to form a complex; and determining the presence or amount of detectable label in the complex or uncomplexed in the reaction medium, wherein the detectable label is a bioluminescent protein.