PLASMODIUM-FALCIPARUM-ANTIGEN OF THE INTRAERYTHROCYTA PHASE, ITS CLEANING AND DETERMINATION OF ANTIQUE AND ANTIBODY AND MALARIA VACCINES CONTAINING IT.

摘要

The invention relates to antigens of the intraerythrocytic stage of Plasmodium falciparum, namely: a 50-kDa protein of isoelectric 5.5 and a 47-kDa protein resolving on electrophoresis into two spots at 5.9 and 6.1 approximately, both proteins appearing in the serum of patients infected with Plasmodium falciparum or in the culture medium of this microorganism during its intraerythrocytic stage, as well as a 126 kDa protein synthesised in the nuclear multiplication stage during schizogony, and localised at the periphery of the schizonts in the vacuole containing the parasite, a precursor of the 50-kDa and 47-kDa as well as 18- and 65-kDa proteins. Use of these proteins in the preparation of malaria vaccines, and of the corresponding monoclonal antibodies in the assay of the antibodies of an immunised subject and in the assay of the antigens according to the invention during an attack of malaria.