ANTIGENIC DETERMINANTS OF AN EIMERIA MEROZOITE SURFACE ANTIGEN VACCINES

摘要

The invention provides a DNA coding for a precursor to an Eimeria merozoite surface antigen, which surface antigen has an apparent molecular weight of about 23 kilodaltons by SDS PAGE. Based on this DNA proteins having one or more immunoreactive and/or antigenic determinants of the said surface antigen may be prepared as well as recombinant vectors and recombinant viruses containing the said DNA or framents thereof and transformed microorganisms containing such vectors and viruses. Thus, the present invention relates also to methods for producing the said proteins and the transformed microorganisms. The present case also relates to the purified 23 kDa merozoite surface antigen itself and the 30 kDa precursor form thereof per se and to methods for protecting poultry against coccidiosis using the Eimeria surface antigen, the precursor protein and/or fragments thereof. The proteins and fragments of the invention can be administered for such protection either as purified proteins or in the form of DNA encoding the protein in a suitable viral vector such as vaccinia virus.