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FUNCTIONALIZED CARRIER MATERIALS FOR THE SIMULTANEOUS SYNTHESIS AND DIRECT LABELLING OF OLIGONUCLEOTIDES AS PRIMERS FOR TEMPLATE-DEPENDENT ENZYMATIC NUCLEIC ACID SYNTHESES
FUNCTIONALIZED CARRIER MATERIALS FOR THE SIMULTANEOUS SYNTHESIS AND DIRECT LABELLING OF OLIGONUCLEOTIDES AS PRIMERS FOR TEMPLATE-DEPENDENT ENZYMATIC NUCLEIC ACID SYNTHESES
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机译:功能性载体材料,用于寡核苷酸的同时合成和直接贴标,作为模板依赖的酶促核酸合成的主要原料
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摘要
The synthesis and use of polymeric carriers is described which are loaded with nucleic acid building blocks which in turn contain labelling groups or precursors thereof. The polymeric carrier loaded in this way serves as a solid or liquid phase for the assembly of oligonucleotides which can be used as primers for template-dependent enzymatic nucleic acid syntheses such as for example in sequencing analysis according to Sanger and co-workers or in the polymerase chain reaction (PCR).
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