Assay to distinguish Corynebacteria and Brevibacteria - by differences in growth and homo-serine dehydrogenase gene fragments for C. glutamicum, B. flavum and B. lactofermentum

摘要

An assay to distinguish Corynebacterium glutamicum (C. glut.), Brevibacterium flavum (B. fla.) and B. lactofermentum (B. Lac.) by the determn. of the optical density after growth (i) in suitable medium without C-source, (ii) with trehalose as a C-source, (iii) pref. in the medium BMCG (Liebl et al, Appl. Microbil. Biotechnology (1989), 32: 205-210) with (ii) or without a C-source, (iv) pref. in either of (i)-(iii), followed by growth on plates contg. ethionine in the medium and by the determn. of colony growth. Also claimed is a detection assay for the presence of 5'-DNA of the hom-gene (homoserine dehydrogenase from C. glut). The assays above can be used individually or in combination with another method. USE/ADVANTAGE - The mentioned bacteria are useful in industry for the prodn. of L-aminoacids. Provides the first method for a taxonomic distinction between the 3 species and any mutants developed can be distinguished