MONOCLONAL ANTIBODY TO INNER SHELL COMMON ANTIGEN OF GROUP C ROTAVIRUS

摘要

PURPOSE: To apply a monoclonal antibody to a process for the quick and easy detection of a group C virus originated from human and animal (LA process, RPHA process and ELISA) by producing a monoclonal antibody to an inner shell common antigen of group C rotavirus originated from human and animal and using the monoclonal antibody reagent. ;CONSTITUTION: A hybridoma capable of forming an essentially uniform monoclonal antibody to an inner shell common antigen of group C rotavirus originated from human and animal (cattle and pig) is produced by the fusion of XAg63 mouse myeloma cell and a spleen cell of a BALB/c mouse immunized with human group C rotavirus. The monoclonal antibody is (a) reactive with essentially all group C rotaviruses originated from human and nonreactive with group A rotavirus, (b) reactive with group C rotavirus originated from cattle or pig and (c) reactive with inner shell common antigen of group C rotavirus originated from human, cattle or pig.;COPYRIGHT: (C)1994,JPO