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METHOD OF INTEGRATING GENE INTO CHROMOSOME OF LACTOBACILLUS DELBRUECKII SPECIES AND PRODUCT OF GENE INTEGRATION
METHOD OF INTEGRATING GENE INTO CHROMOSOME OF LACTOBACILLUS DELBRUECKII SPECIES AND PRODUCT OF GENE INTEGRATION
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机译:将基因整合入德氏乳杆菌属物种的染色体中的方法及基因整合产物
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摘要
A product of gene integration prepared by integrating an arbitrary gene into a chromosomal DNA of a bacillus of Lactobacillus delbrueckii species through a method which comprises constructing an integration plasmid by combining pAMβ1 plasmid with a target gene, subjecting the obtained plasmid to conjugation transfer and homologous recombination to thereby integrate only the target gene into the chromosomal DNA, and eliminating the DNA sequence of the pAMβ1 as a vector. The product can be widely applied to the production of food, drug and feed.
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