STRAIN OF HYBRID CULTURED MOUSE CELLS MUS MUSCULUS USED FOR PREPARING OF MONOCLONAL ANTIBODIES TO THE GROUP-SPECIFIC ANTIGEN IN HUMAN ERYTHROCYTES

摘要

FIELD: biotechnology. SUBSTANCE: strain is prepared by hybridization of spleen cells of mouse Balb/c immunized with human erythrocytes of group B (III) with myeloma cells RZ-X63-Ag 8.653. Productive clones are designated as D2. Strain is stored in Collection of transplanted cell lines in Institute of Hematology and Blood Transfusion at number B-1/85. Cells of strain grow as stationary suspension on the medium RPMI-1640 or DMEM with 20% fetal calf serum, modal chromosome number is 85, cells have two metacentric chromosomes and a single acrocentric chromosome. Titer of monoclonal antibodies in cultural and ascitic fluid is 1:128-1:1024 and 1:512000, respectively. Antibodies defect antigen B in erythrocytes of blood groups B (III) and AB (IV). Activity of antibodies to cultural fluid is 6-10 s. EFFECT: preparing of strain indicated above. 1 tbl