A photo detection system for the dna - bases - analysis.

摘要

PURPOSE:To detect a slight quantity of a fluorescent label DNA with high accuracy by utilizing fluorescent light emission in a wavelength region where gel light emission is small. CONSTITUTION:The light emitted from the gel is smaller as the excitation wavelength and emission wavelength are emitted from the impurity in the gel stock and the light generated by polymn. of acrylamide. The latter is larger as the excitation wavelength is shorter, but said light decreases with an increase in the wavelength and is extremely small at =(530-540nm) excitation wavelength. The detection of the DNA fragment with high sensitivity is, therefore, permitted by labeling the DNA by using a laser having =(530-540nm) wavelength as an excitation light source and using a fluorescent dye having about 600nm emission wavelength or the emission wavelength larger than said length.