Stromal cell lines from human bone marrow cells - contain SV-40 DNA with defective origin of replication, useful as feeder cells, for prodn. of growth factors and for gene expression

摘要

New stromal cell line from human bone marrow contains in its genome DNA sequences from Simian virus (SV)-40 having a defective origin of replication. Partic. a part of the late SV-40 gene encoding the coat protein is deleted, and the virus sequences contain at least a region encoding the T-antigen. Specified cell lines have been deposited as DSm ACC 2055 and 2056. USE/ADVANTAGE - The cell lines are used (1) as feeder layers of haematopoietic cells and osteoclast precursors (e.g. for analysing the effects of drugs on malignant bone marrow cells from leukaemia patients), (2) for prodn. of growth factors (G-CSF and IL-6) and (3) for expressing genes, cloned in vectors which replicate under control of the large T-antigen of SV-40. Because the origin of replication is defective, spontaneous changes in the cell line caused by virus prodn. cannot occur. Unlike prim. stroma, these cell lines have a precisely defined, uniform cell population not subject to experimental variation. They are capable of unlimited division, remain adherent after irradiation and produce large amts. of growth factors (controllable by irradiation or stimulation with interleukin-1).