A yeast strain for co-expressing a heterologous cytochrome P450 monooxygenase activity and an endogenous or heterologous NADPH-cytochrome P450-reductase and its use for bioconversion purposes.

摘要

A yeast strain for the co-expression of a plant cytochrome P450 mono-oxygenase activity and an endogenous or heterologous NADPH-cytochrome P450-reductase, wherein one of the NADPH-cytochrome P450-reductase or cytochrome P450 genes is integrated into the chromosome of said strain, and the strain is transformed by a vector having an expression cassette for the other gene. The use of said yeast strain, as well as a cDNA sequence coding for cytochrome P450 CA4H of the Jerusalem artichoke Helianthus tuberosus or Arabidopsis thaliana, for bioconversion purposes, is also disclosed.