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Recombinant DNA sequences encoding signal peptides, selective interacting polypeptides and membrane anchoring sequences
Recombinant DNA sequences encoding signal peptides, selective interacting polypeptides and membrane anchoring sequences
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机译:编码信号肽,选择性相互作用多肽和膜锚定序列的重组DNA序列
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摘要
Tripartite recombinant DNA encoding fusion proteins which comprise three sequences, i.e., a signal peptide which is operable in a Gram positive bacterium, an immunogenic polypeptide linked thereto which is not normally expressed in a Gram positive bacterium, and a cell wall spanning and a membrane anchoring sequence, as well as their use in Gram positive bacteria to express the resultant fusion protein on their surface are described. The preferred cell wall spanning and anchoring polypeptides include Staphylococcus protein A and Streptococcus protein G.
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