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Controlling perfusion rates in continuous bioreactor culture of animal cells
Controlling perfusion rates in continuous bioreactor culture of animal cells
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机译:在动物细胞连续生物反应器培养中控制灌注速率
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摘要
The invention is a method for controlling the perfusion rate of bioreactor cultures of animal cells, wherein the level of metabolic activity of the cells, as reflected by a drop in pH and as measured by a pH probe, is used to activate 1) the perfusion of fresh medium containing standard cell nutrients and experimentally determined concentrations of energy source(s) and buffer, into the bioreactor and 2) the removal of culture fluid, which is depleted of nutrients and contains waste products, from the bioreactor.
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