Method for preparing platelet factors and device for activation of thrombocytes of human or animal origin. This method for obtaining a solution of platelet factors comprises a step of thrombocyte activation by bringing the thrombocytes in contact with a thrombocyte activating solution, and a solution of platelet factors released by the thrombocytes during the activation stage is collected. The method is characterized in that a liquid containing a suspension of thrombocytes is passed over a filter capable of retaining the thrombocytes, in that an activating solution is added to the thrombocytes retained on the filter, and in that a filtrate containing the platelet factors in solution is separated by filtration, while the thrombocytes remain retained on the filter.
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