STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF ENDONUCLEASE RESTRICTION CFRBI

摘要

FIELD: biotechnology. SUBSTANCE: proposed strain of Escherichia coli BKM CR-370D is prepared by transformation with plasmid DNA pBGM3 containing genes of restriction-modification CFRBI in strain E.coli Z85. Strain provides high level of enzyme content, 5 mln. U/g wet matter of cell biomass. The yield of purified enzyme is 200000 U/g cell biomass. EFFECT: increased yield of enzyme.