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STRAIN OF BACTERIUM BACILLUS SUBTILIS - A PRODUCER OF HYDROLYTIC ENZYME COMPLEX ENRICHED WITH amp;beta;-GLUCANASE
STRAIN OF BACTERIUM BACILLUS SUBTILIS - A PRODUCER OF HYDROLYTIC ENZYME COMPLEX ENRICHED WITH amp;beta;-GLUCANASE
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机译:芽孢杆菌芽孢杆菌的菌株-富含β-葡聚糖酶的水解酶复合物的生产者
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摘要
FIELD: biotechnology, agriculture, food industry, brewery. SUBSTANCE: method involves multiple sowing the strain B. subtilis ВКПМ-13-5165, isolation and purification of colonies on the media containing lichenan as a nutrition source, and the strain B. subtilis ВКПМ B-5790 is obtained. The strain B. subtilis is cultured on the nutrient medium containing brewery dregs, БВК, 1% KH2PO4,, pH 6.8-7.2, cultivation temperature is 33-37 C, cultivation time is 266-32 hr. Cultural fluid has the following enzymatic activities: 1,3-1,4-β-glucanase, 1,3-1,4-b-glucosidase, b-endoglucanase, 1,4-mannanase, xylanase (total cytolytic activity) 20-30 U/ml a-glucoamylase, neutral, alkaline and acid proteases. EFFECT: isolation of bacterial strain indicated above.
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机译:领域:生物技术,农业,食品工业,啤酒厂。实质:该方法涉及对枯草芽孢杆菌ВКПМ-13-5165进行多次播种,在含有地衣聚糖作为营养源的培养基上分离和纯化菌落,从而获得了枯草芽孢杆菌ВКПМB-5790。枯草芽孢杆菌菌株在含有啤酒渣,1%KH 2 Sub> PO 4 Sub>,pH 6.8-7.2的啤酒渣的营养培养基上培养,培养温度为33-37 C,培养时间是266-32小时。培养液具有以下酶活性:1,3-1,4-β-葡聚糖酶,1,3-1,4-b-葡糖苷酶,b-内葡聚糖酶,1,4-甘露聚糖酶,木聚糖酶(总细胞溶解活性)20- 30 U / mlα-葡糖淀粉酶,中性,碱性和酸性蛋白酶。效果:分离上述细菌菌株。
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