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Transient expression system for the production of recombinant protein.
Transient expression system for the production of recombinant protein.
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机译:用于生产重组蛋白的瞬时表达系统。
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摘要
A method is described for transient production of a desired heterologous protein comprising: transfecting a eukaryotic host cell with a vector producing a trans-activating protein; transfecting the eukaryotic host cell with an expression vector comprising a stabilizing sequence downstream of a promoter and upstream of a DNA encoding the desired heterologous protein and a polyadenylation sequence downstream of which is a transcription terminatein site; culturing the transfected eukaryotic host cell under conditions favorable for production of said desired heterologous protein; and, recovering the desired protein in useful amounts within about one day to about fourteen days of transfection.
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