首页>
外国专利>
Nucleic acid analogue-induced transcription of RNAfrom a double-stranded DNA template
Nucleic acid analogue-induced transcription of RNAfrom a double-stranded DNA template
展开▼
机译:核酸类似物诱导的双链DNA模板RNA转录
展开▼
页面导航
摘要
著录项
相似文献
摘要
RNA is transcribed from a double stranded DNA template by forming a complex by hybridizing to the template at a desired transcription initiation site one or more oligonucleic acid analogues of the PNA type capable of forming a transcription initiation site with the DNA and exposing the complex to the action of a DNA dependant RNA polymerase in the presence of nucleoside triphosphates. Equal length transcripts may be obtained by placing a block to transcription downstream from the initiation site or by cutting the template at such a selected location. The initiation site is formed by displacement of one strand of the DNA locally by the PNA hybridization.
展开▼