Manner and system for producing where in the gram positive bacteriums the protein outside the important fungus body is raised commercially

摘要

(57) Abstract This invention conspecific it is excessively produced in the gram positive bacterium like the Bacillus genus bacteriums or offers the manner and revelation system in order the secretion of the protein outside the cell of different kind to raise. As for this manner and system, the fact that the PrsA protein is produced excessively in the gram positive bacterial host who at least can produce the protein outside one intended cell excessively is included. It can raise the secretion to in the breeding nutrient medium of the protein outside the cell which is synthesized by the manner of this invention and using system, substantially. When it is secreted once in the breeding nutrient medium, easily you can recover and can refine the protein outside these cells.