BIOASSAY FOR WILLEBRAND DISEASE

摘要

PURPOSE: To realize bioassay of type 1-3 gold type Willebrand factor (VWF) by adding refined bothrocetin as well as refined ristocetin. ;CONSTITUTION: A refine bothrocetin is an essential element of bioassay for VWF disease and naturally extracted from the venom of rattlesnake in Brazil and refined or obtained through DNA rearrangement. The refine bothrocetin is supplied and brought into contact with the serum or plasma of a patient. VWF in the serum or plasma reacts on the bothrocetin to cause platelet aggregation. The extent of platelet aggregation indicates the presence of VWF in the serum or plasma quantitatively thus providing an index of VWF disease. The extent of platelet aggregation can be determined visually using an appropriate optical machine. The bioassay specific to type 2 of VWF includes a process for bringing a part of the serum or plasma into contact with bothrocetin and then bringing another part thereof into contact with refined ristocetin before comparing the extent of platelet aggregation between them.;COPYRIGHT: (C)1996,JPO