METHOD FOR DIAGNOSING THEILERIA SERGENTI FOR EACH DNA TYPE THEREOF

摘要

PURPOSE: To discriminate the types of the Theileria sergenti which are different on the points of the DNA and the antigenicity and which are contained in a protozoan strain, by creating a primer capable of specifically multiplying three types of I type, C type and B type Theileria sergenti strains and subsequently subjecting the created primer to a PCR. ;CONSTITUTION: Primers, (I) 5'-AAGGATCCGTCTCTGCTACCGCGC-3', (II) 5'-GCGGATCCTCATCGTCTCTGCAACT-3' and (III) 5'- GCGGATCCGCTCTGCAACCGCAGAG-3', capable of specifically multiplying Theileria sergenti strains, I type, C type and B type, are combined with Ts-R primer 5'-TGTGAGACTCAATGCGCCTA-3' and subsequently subjected to a PCR.;COPYRIGHT: (C)1996,JPO