VARIANT POLYPEPTIDES OF HUMAN KUNITZ TYPE PROTEASE INHIBITOR DOMAIN 1 OF TISSUE FACTOR PATHWAY INHIBITOR AND COMPOSITIONS THEREOF

摘要

A variant of human Kunitz-type protease inhibitor domain I of tissue factor pathway inhibitor (TFPI), the variant comprising the following amino acid sequence X1 Cys Ala Phe Lys Ala Asp X2 Gly X3 Cys X4 X5 X6 X7 X8 X9 Phe Phe Phe Asn Ile Phe Thr Arg Gln Cys Glu Glu Phe X10 Tyr Gly Gly Cys X11 X12 X13 Gln Asn Arg Phe X14 Ser Leu Glu Glu Cys X15 X16 Met Cys Thr Arg X17 (SEQ ID No. 1), wherein X1 represents H or 1-7 naturally occurring amino acid residues except Cys, X2-X16 each independently represents a naturally occurring amino acid residue, and X17 represents OH or 1-5 naturally occurring amino acid residues except Cys, with the proviso that at least one of the amino acid residues X1-X17 is different from the corresponding amino acid residue of the native sequence.