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SECRETION OF PROTEINS FROM THE OUTER MEMBRANE OF GRAM-NEGATIVE BACTERIA BY GRAM-POSITIVE HOST ORGANISMS
SECRETION OF PROTEINS FROM THE OUTER MEMBRANE OF GRAM-NEGATIVE BACTERIA BY GRAM-POSITIVE HOST ORGANISMS
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机译:革兰氏阳性菌从革兰氏阴性菌外膜分泌蛋白
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PCT No. PCT/DE94/01394 Sec. 371 Date Jul. 18, 1996 Sec. 102(e) Date Jul. 18, 1996 PCT Filed Nov. 21, 1994 PCT Pub. No. WO95/20048 PCT Pub. Date Jul. 27, 1995The invention relates to a process for producing outer membrane proteins of gram-negative bacteria by gram-positive host cells. To this end, a gene structure containing a gene coded for an outer membrane protein is constructed; in front of this gene there is a pro-gene sequence which codes for a propeptide of an export protein of a gram-positive bacterium. In front of the pro-gene sequence there is a pre-gene sequence which codes for a signal peptide of an export protein of a gram-positive bacterium. After the cloning of a gene structure thus constructed into a vector and transformation into a gram-positive bacterium, the outer membrane protein is expressed with a signal peptide and propeptide, transported through the membrane of the gram-positive host cell and released into the test of the culture.
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