NON-DENATURING POTENCY ASSAY FOR BOVINE SOMATOTROPIN to measure the efficacy of bovine growth hormone

摘要

The present invention describes a method for measuring the efficacy of bovine growth hormone. The content of biologically active bovine growth hormone in bovine growth hormone samples is a hydrophilic, porous polymer gel having an average particle diameter of about 5 μm to about 15 μm as a stationary phase and a pH of about 8 to about 12 as a mobile phase and a growth of bovine Hormone samples are measured by size exclusion HPLC using an aqueous buffer solution that does not denature. The efficacy of a bovine growth hormone sample is determined based on the measurand of the biologically active bovine growth hormone protein thus determined. This method provides a convenient, precise and accurate method for measuring the efficacy of bST samples.